Composite

Part:BBa_K4773011

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


Lac Promoter+RBS+NicA+RBS+NicB+RBS+NicC+Term

This part is a gene combination consisting of Lac Promoter+RBS+NicA+RBS+NicB+RBS+NicC+Term, which can express three enzymes involved in nicotine degradation under lactose induction. Lac Promoter is a promoter from Escherichia coli, which can activate the transcription of downstream genes in the presence of lactose. RBS is a strong bacterial ribosome binding site (BBa_B0034), which can ensure the efficient translation of each gene. NicA, NicB and NicC are three enzymes from Pseudomonas putida S16, which can degrade nicotine to succinate and glycine. Term is a double terminator (BBa_B0015), which can prevent read-through transcription and ensure the stability of the mRNA. This part can be used to construct biological systems for nicotine degradation or bioremediation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1421
    Illegal BglII site found at 1662
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None