Translational_Unit

Part:BBa_K4773010

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


RBS-ATF1

This part is a gene encoding the ATF1 enzyme from Saccharomyces cerevisiae, which can catalyze the formation of volatile acetate esters from acetyl-CoA and alcohols during fermentation. This part can be used to modulate the flavor and aroma of fermented products or to produce biofuels. The part also contains a strong bacterial ribosome binding site (RBS) (BBa_B0034) to ensure efficient translation of the ATF1 gene. There are SacI and SalI restriction sites at the end of the ATF1 gene to facilitate cloning into standard plasmid vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 117
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544
    Illegal SapI.rc site found at 1552


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Parameters
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