Translational_Unit

Part:BBa_K4773009

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


RBS-AprBA

This part is a gene encoding the adhE2 enzyme from Clostridium acetobutylicum, which can catalyze the reversible conversion of butyraldehyde and NADH to butanol and NAD+ in the presence of CoA. This part can be used to construct biocatalysts for butyrate utilization or biofuel production. The part also contains a strong bacterial ribosome binding site (RBS) (BBa_B0034) to ensure efficient translation of the adhE2 gene. A HindIII restriction site was added at the end of the adhE2 gene to facilitate cloning into standard plasmid vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1476
    Illegal XhoI site found at 2596
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1813
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None