Translational_Unit

Part:BBa_K4773007

Designed by: Yan Pan   Group: iGEM23_UESTC-China   (2023-10-01)


RBS-buk

This part is a gene encoding the buk enzyme from Clostridium acetobutylicum, which can degrade butyrate to acetyl-CoA and butyryl-CoA in the presence of NAD+ and CoA. This part can be used to construct biocatalysts for butyrate utilization or biofuel production. The part also contains a strong bacterial ribosome binding site (RBS) (BBa_B0034) to ensure efficient translation of the buk gene. A HindIII restriction site was added at the end of the buk gene to facilitate cloning into standard plasmid vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 540
    Illegal BglII site found at 681
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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