DNA

Part:BBa_K4769306

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


rspD promoter 3’ integration sequence for B. subtilis 3610

Integration sequence for homologous recombination into the genome, proximal to the promoter of the rpsD gene (essential, encodes ribosomal protein S4) of B. subtilis 3610

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 935
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 935
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 935
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 935
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None