DNA
Part:BBa_K4769304
Designed by: Kieran Abbott Group: iGEM23_Cambridge (2023-10-10)
gyrA promoter 3’ integration sequence for B. subtilis 3610
Integration sequence for homologous recombination into the genome, proximal to the promoter of the gyrA gene (essential, encodes subunit A of DNA gyrase) of B. subtilis 3610
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 642
Illegal AgeI site found at 254 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 225
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Categories
Parameters
None |