DNA

Part:BBa_K4769303

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


spoIIE knockout 3’ integration sequence for B. subtilis 3610

Integration sequence for homologous recombination to remove the spoIIE gene (encodes a phosphatase involved in spore formation) of B. subtilis 3610

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 748
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 748
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 748
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 748
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 415


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Categories
Parameters
None