DNA
Part:BBa_K4769303
Designed by: Kieran Abbott Group: iGEM23_Cambridge (2023-10-10)
spoIIE knockout 3’ integration sequence for B. subtilis 3610
Integration sequence for homologous recombination to remove the spoIIE gene (encodes a phosphatase involved in spore formation) of B. subtilis 3610
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 748
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 748
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 748
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 748
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 415
[edit]
Categories
Parameters
None |