Coding

Part:BBa_K4769212

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


nprB coding sequence from B. subtilis 3610

Coding sequence of the nprB gene from B. subtilis 3610; encodes the extracellular neutral protease, neutral protease B (see DOI: 10.1371/journal.pgen.1008232)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 320
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 320
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 320
    Illegal BamHI site found at 270
    Illegal XhoI site found at 472
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 320
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 320
    Illegal NgoMIV site found at 34
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None