Coding

Part:BBa_K4769207

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


eps operon coding sequence from B. subtilis 3610

Coding sequence of the eps operon from B. subtilis 3610; involved in biosynthesis of polysaccharides of the biofilm extracellular polymeric substances (see DOI: 10.1111/mmi.12697)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2173
    Illegal PstI site found at 410
    Illegal PstI site found at 1519
    Illegal PstI site found at 1868
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2173
    Illegal PstI site found at 410
    Illegal PstI site found at 1519
    Illegal PstI site found at 1868
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2173
    Illegal BglII site found at 1580
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2173
    Illegal PstI site found at 410
    Illegal PstI site found at 1519
    Illegal PstI site found at 1868
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2173
    Illegal PstI site found at 410
    Illegal PstI site found at 1519
    Illegal PstI site found at 1868
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1357
    Illegal SapI.rc site found at 1525


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Parameters
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