Coding
Part:BBa_K4769205
Designed by: Kieran Abbott Group: iGEM23_Cambridge (2023-10-10)
yqxM-sipW-tasA operon coding sequence from B. subtilis 3610
Coding sequence of the yqxM-sipW-tasA operon from B. subtilis 3610; involved in production of tasA, the major protein component of the biofilm extracellular polymeric substances (see DOI: 10.1111/mmi.12697)
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1531
Illegal PstI site found at 1419 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1531
Illegal PstI site found at 1419 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1531
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1531
Illegal PstI site found at 1419 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1531
Illegal PstI site found at 1419
Illegal NgoMIV site found at 841
Illegal NgoMIV site found at 1142 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 448
Illegal BsaI.rc site found at 1032
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Categories
Parameters
None |