Coding

Part:BBa_K4769205

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


yqxM-sipW-tasA operon coding sequence from B. subtilis 3610

Coding sequence of the yqxM-sipW-tasA operon from B. subtilis 3610; involved in production of tasA, the major protein component of the biofilm extracellular polymeric substances (see DOI: 10.1111/mmi.12697)

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1531
    Illegal PstI site found at 1419
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1531
    Illegal PstI site found at 1419
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1531
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1531
    Illegal PstI site found at 1419
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1531
    Illegal PstI site found at 1419
    Illegal NgoMIV site found at 841
    Illegal NgoMIV site found at 1142
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 448
    Illegal BsaI.rc site found at 1032


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Categories
Parameters
None