DNA

Part:BBa_K4769110

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


Porphyran utilisation locus fragment 4 integration sequence

Integration sequence for homologous recombination into the 3’ end of PUL fragment 4 (for inserting fragment 5) in a B. subtilis strain containing porphyran utilisation locus fragment 4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 909
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 747


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Categories
Parameters
None