DNA

Part:BBa_K4769100

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


amyE 5’ integration sequence for B. subtilis 3610

Integration sequence for homologous recombination into the amyE gene (encodes α-amylase) of B. subtilis 3610

Integration can be confirmed by a starch hydrolysis test (B. subtilis with disrupted amyE is unable to hydrolyse starch)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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