DNA

Part:BBa_K4765133

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-08)


ribozyme connected: intimin-Nb3 + mScarlet

contributed by Fudan iGEM 2023

Usage and Biology

This composite part is the version of BBa_K4765106 with mScarlet added. We use this part to observe the biofilm formation process.

Characterization

Get details in BBa_K4765106.

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From left lane(1) to right lane(2) indicate the successful construction of intimin-Nb3 and intimin-Nb3 + mScarlet.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 2967
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1305
    Illegal BsaI.rc site found at 2201


[edit]
Categories
//collections/biofilm
Parameters
proteins