Plasmid_Backbone

Part:BBa_K4765027

Designed by: Siliang Zhan   Group: iGEM23_Fudan   (2023-10-10)


pET28a modified

contributed by Fudan iGEM 2023

Usage and Biology

pET28a is a plasmid backbone with KanR, we constructed all the ribozyme connected parts with it.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 402
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 402
    Illegal BglII site found at 1
    Illegal BglII site found at 513
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 402
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 402
    Illegal NgoMIV site found at 3555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 1428


[edit]
Categories
Parameters
None