Primer

Part:BBa_K4761513

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-10-05)


gibson-ARO10-rPCR-F

Forward primer for creating backbone of pCDFDuet or pETDuet or pACYCDuet vector when trying to install ARO10-adh6 gene using Gibson Assembly. The three vectors share one primer for they share the same sequence in this range.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None