Primer

Part:BBa_K4761503

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-10-05)


pET-KpnI-R

Reverse primer for creating backbone of pET plasmid. Adds KpnI restriction site on its end.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 22
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 22
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 22
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None