DNA

Part:BBa_K4761240

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-30)


TnL-ARO10-TnR

ARO10 knock-in transposon unit. Installed into pDonor using Gibson® Assembly.

This Composite Part consists of Transposon Left End (BBa_K4761050), ARO10 (BBa_K4761000), and Transposon Right End (BBa_K4761051). It is a transposon containing the ARO10 gene, located on the pDonor plasmid. The pDonor plasmid carrying this transposon is named pDonor-ARO10. In the CRISPR RNA-guided integrase system-mediated integration of the ARO10 gene, it serves as the donor DNA for the ARO10 gene.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1942
    Illegal SpeI site found at 893
    Illegal PstI site found at 5
    Illegal PstI site found at 771
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1942
    Illegal SpeI site found at 893
    Illegal PstI site found at 5
    Illegal PstI site found at 771
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1942
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1942
    Illegal SpeI site found at 893
    Illegal PstI site found at 5
    Illegal PstI site found at 771
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1942
    Illegal SpeI site found at 893
    Illegal PstI site found at 5
    Illegal PstI site found at 771
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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