Generator

Part:BBa_K4761220

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-30)


adh6-linker1-ARO10

adh6-linker1-ARO10 fusion expression unit for tyrosol production. Because the reaction catalyzed by yeast-derived pyruvate decarboxylase ARO10 and endogenous alcohol dehydrogenase ADH6 is an adjacent reaction in salidroside metabolism, the adh6 gene and ARO10 gene are linked through the linker designed by us. The link was made using a fusion PCR method, in which the stop codon of the adh6 gene was replaced with a linker sequence.

For guidelines for fusion PCR and guidelines for its primer design, see BBa_K4761222.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2885
    Illegal SpeI site found at 1836
    Illegal PstI site found at 1714
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2885
    Illegal SpeI site found at 1836
    Illegal PstI site found at 1714
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2885
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2885
    Illegal SpeI site found at 1836
    Illegal PstI site found at 1714
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2885
    Illegal SpeI site found at 1836
    Illegal PstI site found at 1714
    Illegal NgoMIV site found at 991
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Characterization of fusion tyrosol generator genes are all went through the same process, for details, see BBa_K4761222.

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Parameters
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