Plasmid

Part:BBa_K4761112

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-10-06)


pET28a-EGFP

Luminescence expression report plasmid pET28a-EGFP. Induces under 0.1% IPTG. Note that it has no antibiotic resistance. Could express very strong luminescence able to observe under naked eye.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5134
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4964
    Illegal BamHI site found at 5167
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 3184
    Illegal NgoMIV site found at 3344
    Illegal NgoMIV site found at 4932
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2263


Results

The plasmid pET28a-EGFP was transferred into BL21(DE3) chassis (with no RNA Thermometers installed). Upon 18h after induction by 0.1% IPTG, the luminescence is clear and visible under naked eye.

Fig.1 Pellet of BL21(DE3) chassis with pET28a-EGFP 18h after IPTG induction.
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Categories
Parameters
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