Plasmid

Part:BBa_K4761111

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-10-06)


pET-EGFP

Luminescence expression report plasmid pET-EGFP. Constructed from EGFP(BBa_K4761005) and pET vector(BBa_K4761101).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 946
    Illegal XhoI site found at 1029
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1346
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1931
    Illegal SapI.rc site found at 3591


Characterization

pET-EGFP is proven to possess luminescence intensity that is hard to observe under naked eye but detectable via microplate readers. See BBa_K4761101.

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Categories
Parameters
None