Plasmid

Part:BBa_K4761109

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-10-01)


pCutamp

pCutamp carries Cas9 gene to eliminate pDonor and pEffector plasmids after gene knock-in succeeds in CRISPR-Transposon system. Could be activated by rhamnose.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1734
    Illegal EcoRI site found at 3243
    Illegal EcoRI site found at 4427
    Illegal EcoRI site found at 5893
    Illegal EcoRI site found at 12397
    Illegal XbaI site found at 431
    Illegal SpeI site found at 9028
    Illegal PstI site found at 2738
    Illegal PstI site found at 2985
    Illegal PstI site found at 9693
    Illegal PstI site found at 10823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1734
    Illegal EcoRI site found at 3243
    Illegal EcoRI site found at 4427
    Illegal EcoRI site found at 5893
    Illegal EcoRI site found at 12397
    Illegal NheI site found at 7602
    Illegal NheI site found at 12156
    Illegal SpeI site found at 9028
    Illegal PstI site found at 2738
    Illegal PstI site found at 2985
    Illegal PstI site found at 9693
    Illegal PstI site found at 10823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1734
    Illegal EcoRI site found at 3243
    Illegal EcoRI site found at 4427
    Illegal EcoRI site found at 5893
    Illegal EcoRI site found at 12397
    Illegal BglII site found at 6573
    Illegal BamHI site found at 1673
    Illegal BamHI site found at 14435
    Illegal XhoI site found at 9927
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1734
    Illegal EcoRI site found at 3243
    Illegal EcoRI site found at 4427
    Illegal EcoRI site found at 5893
    Illegal EcoRI site found at 12397
    Illegal XbaI site found at 431
    Illegal SpeI site found at 9028
    Illegal PstI site found at 2738
    Illegal PstI site found at 2985
    Illegal PstI site found at 9693
    Illegal PstI site found at 10823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1734
    Illegal EcoRI site found at 3243
    Illegal EcoRI site found at 4427
    Illegal EcoRI site found at 5893
    Illegal EcoRI site found at 12397
    Illegal XbaI site found at 431
    Illegal SpeI site found at 9028
    Illegal PstI site found at 2738
    Illegal PstI site found at 2985
    Illegal PstI site found at 9693
    Illegal PstI site found at 10823
    Illegal NgoMIV site found at 10611
    Illegal AgeI site found at 1508
    Illegal AgeI site found at 3165
    Illegal AgeI site found at 6305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1490
    Illegal SapI site found at 9870


[edit]
Categories
Parameters
None