DNA

Part:BBa_K4761028

Designed by: Leidun Xu   Group: iGEM23_HiZJU-China   (2023-09-29)


sgRNA(pykF)4

The sgRNA designed for knockout of pykA and is used with the plasmid pTarget(BBa_K4761107). The complete sgRNA(pykF)4 expression box can be constructed by connecting sgRNA(pykF)4 to plasmid pTarget by rPCR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 13
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 13
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 13
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 13
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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