DNA

Part:BBa_K4761023

Designed by: Leidun Xu   Group: iGEM23_HiZJU-China   (2023-09-29)


sgRNA(pykA)2

The sgRNA designed for knockout of pykA and is used with the plasmid pTarget(BBa_K4761107). The complete sgRNA(pykA)2 expression box can be constructed by connecting sgRNA(pykA)2 to plasmid pTarget by rPCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None