DNA

Part:BBa_K4761021

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-28)


sgRNA(pheA)2

The sgRNA designed for knockout of pheA and is used with the plasmid pTarget(BBa_K4761107). The complete sgRNA(pheA)2 expression box can be constructed by connecting sgRNA(pheA)2 to plasmid pTarget by rPCR.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None