Coding

Part:BBa_K4761000

Designed by: Pinhan Pan   Group: iGEM23_HiZJU-China   (2023-09-27)


ARO10

Coding for phenylpyruvate decarboxylase in S.cerevisiae. Capable of forming 4-HPAA from 4-HPP in rhodioloside synthesis pathway. Enables phenylpyruvate decarboxylase activity. Involved in cellular amino acid catabolic process. Located in cytosol.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1791
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1791
    Illegal SpeI site found at 742
    Illegal PstI site found at 620
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

In 2023 HiZJU-China, ARO10 is characterized along with adh6 as a whole independent unit. See ARO10-adh6(BBa_K4761200).

[edit]
Categories
Parameters
None