Composite

Part:BBa_K4759206

Designed by: Jianghua Chen   Group: iGEM23_Jiangnan-China   (2023-10-07)


T7-RBS4-TF-linker-OleP

We add a TF(solubilization-promoting tag) at the N-terminal of the P450 enzyme

Usage and Biology

We add a solubilization-promoting tag at the N-terminal of the P450 enzyme, and in the selected six solubilization-promoting tags (SUMO, GST, MBP, TF, TRX, Nus), MBP and TF can significantly improve the solubility of Olep protein, but the enzyme activity of Olep is reduced, and the catalytic efficiency is lower than strain without solubilizing tag.


protein-tags.png

Fig1: The application of protein tags

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 591
    Illegal AgeI site found at 1428
    Illegal AgeI site found at 1526
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 159
    Illegal SapI.rc site found at 807
    Illegal SapI.rc site found at 920
    Illegal SapI.rc site found at 1176


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