Coding

Part:BBa_K4759031

Designed by: Mengrui Tao   Group: iGEM23_Jiangnan-China   (2023-10-02)


T7-RBS4

We used pETDuet / pRSFDuet as a plasmid vector and expressed OleP / GFP11-linker-OleP and a highly soluble tag under the control of T7-RBS 4. The nadK was expressed by the pACYCDuet plasmid vector under the control of T7-RBS 4. Using pETDuet as the plasmid vector, we expressed hemB / hemD / hemC under the control of T7-RBS 4

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None