Part:BBa_K4759015
SUMO
Recombinant protein expression plays an important role in promoting the development of modern biological technology. By using the host to achieve exogenous protein expression, it provides a powerful tool for studying the structure and function of some important proteins, especially the human source proteins. In order to enable subsequent structure-function studies, the obtained recombinant proteins must be soluble, stable, properly folded as well as biologically active. However, in practical studies, these often become obstacles to obtaining an ideal recombinant protein. The solubility of the recombinant protein expression became the primary addressed problem.Small ubiquitin-related modifier (SUMO) are extremely common in eukaryotes and are not found in prokaryotes. The biggest characteristic of SUMO tag is that it can be specifically recognized by SUMO protease and highly degraded, making the subsequent tag removal process accurate and efficient. The SUMO tag can not only enhance the solubility of the recombinant protein, but also increase its expression level. The wild-type SUMO tag cannot be applied in a eukaryotic expression system because of the endogenous SUMO protease in eukaryotic cells. LifeSensors The company has developed an engineered SUMO tag and a supporting specific protease, enabling the SUMO tag to be applied to eukaryotic expression systems such as yeast, insect cells and mammalian cells.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 209
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 209
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 209
Illegal BglII site found at 98 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 209
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 209
- 1000COMPATIBLE WITH RFC[1000]
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