Composite
Part:BBa_K4757070
Designed by: Marik Müller Group: iGEM23_Heidelberg (2023-12-14)
Constructs for testing of different sRNAs targeting mKate2 and/or the RBS BBa_J61101
Construct for testing different sRNA's targeting either mKate2 or the RBS BBa_J61101. Sequences encoding the sRNA's can be cloned via Golden Gate assembly with SapI. The sRNA expression is controlled by the XylS/Pm inducible expression system. mKate2 is expressed by the constitutive promoter pEM7. For more information visit our Wiki: iGEM Heidelberg 2023 RemixHD or our special prize composite part BBa_K4757999
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1110
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 388
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 925
Illegal SapI site found at 1255
Illegal SapI.rc site found at 1229
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Categories
Parameters
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