Composite

Part:BBa_K4757070

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-12-14)


Constructs for testing of different sRNAs targeting mKate2 and/or the RBS BBa_J61101

Construct for testing different sRNA's targeting either mKate2 or the RBS BBa_J61101. Sequences encoding the sRNA's can be cloned via Golden Gate assembly with SapI. The sRNA expression is controlled by the XylS/Pm inducible expression system. mKate2 is expressed by the constitutive promoter pEM7. For more information visit our Wiki: iGEM Heidelberg 2023 RemixHD or our special prize composite part BBa_K4757999

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 925
    Illegal SapI site found at 1255
    Illegal SapI.rc site found at 1229


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Categories
Parameters
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