Composite

Part:BBa_K4757066

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-11-16)


pEM7-AlkS-V760E_pAlkB-RBS-mKate2

Construct used to asses functionality of AlkS-V760E. mKate2 expression is regulated by the AlkS/pAlkB inducible expression system. AlkS expression is promoted by the constitute promoter pEM7. For more information visit our wiki or composite part BBa_K4757999

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1784
    Illegal EcoRI site found at 1880
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1784
    Illegal EcoRI site found at 1880
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1784
    Illegal EcoRI site found at 1880
    Illegal BamHI site found at 61
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1784
    Illegal EcoRI site found at 1880
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1784
    Illegal EcoRI site found at 1880
    Illegal NgoMIV site found at 298
    Illegal NgoMIV site found at 769
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None