Composite
Part:BBa_K4757064
Designed by: Marik Müller Group: iGEM23_Heidelberg (2023-11-16)
Ps1/Ps2-XylS/Pm-RBS1-SPpstu-FAST-PETase
Expression and secretion of the FAST-PETase and subsequent secretion into the extracellular media by the SPpstu secretion tag. Expression is controlled by the XylS/Pm expression system, inducible with 3-methyl-benzoate. The XylS expression is controlled by the Ps1/Ps2 promoter system. For more information visit our wiki RemixHD.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1110
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 388
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 925
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Categories
Parameters
None |