Composite

Part:BBa_K4757064

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-11-16)


Ps1/Ps2-XylS/Pm-RBS1-SPpstu-FAST-PETase

Expression and secretion of the FAST-PETase and subsequent secretion into the extracellular media by the SPpstu secretion tag. Expression is controlled by the XylS/Pm expression system, inducible with 3-methyl-benzoate. The XylS expression is controlled by the Ps1/Ps2 promoter system. For more information visit our wiki RemixHD.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1110
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 388
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 925


[edit]
Categories
Parameters
None