Coding

Part:BBa_K4757015

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-10-03)


FAST-PETase

The FAST-PETase (functional, active, stable and tolerant PETase) is an engineered IsPETase that contains five point mutations (S121E, D186H, N233K, R224Q, R280A). The FAST-PETase shows increased stability and activity at mesophilic temperatures, compared to other engineered IsPETases).

The sequence was codon-optimized for Pseudomonas-Putida, using the IDT codon optimization tool)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None