Coding
Part:BBa_K4757013
Designed by: Marik Müller Group: iGEM23_Heidelberg (2023-10-03)
AlkS-epS4-156
The wildtype (WT) AlkS transcription factor can be positively regulated with alkanes with a length up to C12. This engineered AlkS with the point mutation V760E was shown to be able to sense alkanes up to C17.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1705
Illegal EcoRI site found at 1801 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1705
Illegal EcoRI site found at 1801 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1705
Illegal EcoRI site found at 1801 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1705
Illegal EcoRI site found at 1801 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1705
Illegal EcoRI site found at 1801
Illegal NgoMIV site found at 219
Illegal NgoMIV site found at 690 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |