Composite

Part:BBa_K4757007

Designed by: Marik Müller   Group: iGEM23_Heidelberg   (2023-11-16)


pEM7 AlkS-V760E/pAlkB

Expression cassette with the constitutive promoter pEM7 and the inducible AlkS-V760E/pAlkB expression system. The EM7 promoter expresses AlkS-V760E. AlkS-V760E is a inducible transcription factor which binds to pAlkB and promotes expression after n-alkane induction. In our experiments we could show induction with n-dodecane. For further informatio refer to our composite part BBa_K4757999 or our wiki

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1753
    Illegal EcoRI site found at 1849
    Illegal NgoMIV site found at 267
    Illegal NgoMIV site found at 738
  • 1000
    COMPATIBLE WITH RFC[1000]


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