Part:BBa_K4752006
Bx
Discription
Bx is a reporter designed by Yuanjie Deng el al. Betaxanthins, a distinct class of plant pigments, impart the striking yellow-orange hue observed in various plant species such as Beta vulgaris, Mirabilis jalapa, Portulaca grandiflora, and Opuntia ficus-indica due to their accumulation. Similar to Betalains, betaxanthins originate from tyrosine and, in addition to their vibrant color, exhibit visible green fluorescence when exposed to blue light excitation. [1]
The intricate biosynthetic pathway of betalains involves key enzymatic components, namely, P450 oxygenase CYP76AD1 and L-DOPA 4,5-dioxynase (DODA). Tyrosine undergoes hydroxylation on the benzene ring catalyzed by the P450 oxygenase CYP76AD1, resulting in L-3,4-dihydroxyphenylalanine (L-DOPA). Subsequently, L-DOPA can be enzymatically catalyzed by DODA to yield betalamic acid. Ultimately, betalamic acid, in conjunction with amines and amino acids, undergoes a transformation process leading to the production of Betaxanthin.[2]
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 85
Illegal PstI site found at 2392 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2392
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 160
Illegal BglII site found at 778 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 85
Illegal PstI site found at 2392 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 85
Illegal PstI site found at 2392 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 515
Illegal SapI.rc site found at 2045
References
- ↑ F. Gandía-Herrero, J. Escribano, F. García-Carmona, Betaxanthins as pigments responsible for visible fluorescence in flowers, Planta 222 (2005) 586–593.
- ↑ Yuanjie Deng, Aoqi Duan, Tong Li, Huiru Wang, Aisheng Xiong, A betaxanthin-based visible and fluorescent reporter for monitoring plant transformation, The Crop Journal, Volume 11, Issue 2 (2023) 666-671,
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