Part:BBa_K4752004

GLR2.9 promoter

Discription

GLR 2.9 promoter is intrinsically associated with plant responses to pests and diseases. In the realm of gene expression regulation, promoters dictate when, where, and how a specific gene is expressed. A notable characteristic of the GLR2.9 promoter is its pronounced activation upon pest and disease infestation, subsequently initiating the expression of relevant genes to assist the plant in mounting a defense.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 630
  Illegal EcoRI site found at 1257
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 630
  Illegal EcoRI site found at 1257
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 630
  Illegal EcoRI site found at 1257
  Illegal BglII site found at 1067
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 630
  Illegal EcoRI site found at 1257
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 630
  Illegal EcoRI site found at 1257
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5
  

Preperation

By extracting the entire genome of Arabidopsis thaliana, we successfully obtained the sequence fragment of the GLR2.9 promoter. To facilitate the assembly of subsequent experiments, we designed a set of primers for both the RUBY and BX systems with the aim of amplifying the promoter from the plant's entire genome via PCR. We conducted gradient experiments to identify the optimal annealing temperature and further verified the results through gel electrophoresis.