Part:BBa_K4747020

araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725-yhjF-PA0727

The yhjH gene, encoding the phosphodiesterase (PDE) of the c-di-GMP pathway, was adeptly integrated within PA0726 aiming to mitigate biofilm formation, under the regulatory ambit of the arabinose operon. This operon, housing the araC gene and araBAD promoter, positions AraC as the regulatory pivot, adroitly coordinating araBAD expression in harmony with arabinose availability.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7209
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7715
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7209
  Illegal NheI site found at 7271
  Illegal NheI site found at 8173
  Illegal PstI site found at 7715
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7209
  Illegal BglII site found at 310
  Illegal BamHI site found at 5505
  Illegal BamHI site found at 5625
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7209
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7715
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7209
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7715
  Illegal NgoMIV site found at 974
  Illegal NgoMIV site found at 3270
  Illegal NgoMIV site found at 3396
  Illegal NgoMIV site found at 3406
  Illegal NgoMIV site found at 5744
  Illegal NgoMIV site found at 6051
  Illegal NgoMIV site found at 7314
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 3298
  Illegal SapI.rc site found at 823
  Illegal SapI.rc site found at 1033
  Illegal SapI.rc site found at 5251