Part:BBa_K4747019

araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725-wspF-PA0727

The wspF gene, a key inhibitor of the c-di-GMP pathway, was adeptly inserted within PA0726 aiming to curb biofilm formation, with its regulation finely tuned by the arabinose operon. This operon, housing the araC gene and araBAD promoter, positions AraC as the regulatory nexus, adroitly coordinating araBAD expression in harmony with arabinose availability.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7452
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7958
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7452
  Illegal NheI site found at 7514
  Illegal NheI site found at 8416
  Illegal PstI site found at 7958
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7452
  Illegal BglII site found at 310
  Illegal BamHI site found at 5505
  Illegal BamHI site found at 5625
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7452
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7958
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7452
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7958
  Illegal NgoMIV site found at 974
  Illegal NgoMIV site found at 3270
  Illegal NgoMIV site found at 3396
  Illegal NgoMIV site found at 3406
  Illegal NgoMIV site found at 5744
  Illegal NgoMIV site found at 6051
  Illegal NgoMIV site found at 6723
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 3298
  Illegal SapI.rc site found at 823
  Illegal SapI.rc site found at 1033
  Illegal SapI.rc site found at 5251