Part:BBa_K4747018

araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725-ytnP-PA0727

The ytnP gene, encoding a quorum-quenching enzyme, was astutely nestled within PA0726, aiming to mitigate biofilm accretion, with its regulation finely tuned by the arabinose operon. The arabinose operon, encompassing the araC gene and araBAD promoter, positions AraC as the regulatory linchpin, adeptly choreographing araBAD expression in harmony with arabinose availability.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7212
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7718
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7212
  Illegal NheI site found at 7274
  Illegal NheI site found at 8176
  Illegal PstI site found at 7718
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7212
  Illegal BglII site found at 310
  Illegal BglII site found at 6966
  Illegal BamHI site found at 5505
  Illegal BamHI site found at 5625
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7212
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7718
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 5484
  Illegal EcoRI site found at 7212
  Illegal XbaI site found at 6382
  Illegal PstI site found at 7718
  Illegal NgoMIV site found at 974
  Illegal NgoMIV site found at 3270
  Illegal NgoMIV site found at 3396
  Illegal NgoMIV site found at 3406
  Illegal NgoMIV site found at 5744
  Illegal NgoMIV site found at 6051
  Illegal NgoMIV site found at 7317
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI site found at 3298
  Illegal SapI.rc site found at 823
  Illegal SapI.rc site found at 1033
  Illegal SapI.rc site found at 5251