DNA

Part:BBa_K4747017

Designed by: Liu Lantian   Group: iGEM23_SUSTech-MED   (2023-10-11)


araC-araBAD-xisF4-PA0717-PA0718-PA0719-PA0720-PA0722-CoaB-PA0724-PA0725-aiiA-PA0727

The gene aiiA, encoding quorum quenching enzyme, was strategically integrated within PA0726 with an objective to attenuate biofilm formation, and its regulation is orchestrated by the arabinose operon. The arabinose operon, encompassing the araC gene and araBAD promoter, features AraC as the regulatory fulcrum, deftly orchestrating araBAD expression in sync with arabinose presence.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7386
    Illegal XbaI site found at 6382
    Illegal PstI site found at 6928
    Illegal PstI site found at 7892
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7386
    Illegal NheI site found at 7448
    Illegal NheI site found at 8350
    Illegal PstI site found at 6928
    Illegal PstI site found at 7892
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7386
    Illegal BglII site found at 310
    Illegal BamHI site found at 5505
    Illegal BamHI site found at 5625
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7386
    Illegal XbaI site found at 6382
    Illegal PstI site found at 6928
    Illegal PstI site found at 7892
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5484
    Illegal EcoRI site found at 7386
    Illegal XbaI site found at 6382
    Illegal PstI site found at 6928
    Illegal PstI site found at 7892
    Illegal NgoMIV site found at 974
    Illegal NgoMIV site found at 3270
    Illegal NgoMIV site found at 3396
    Illegal NgoMIV site found at 3406
    Illegal NgoMIV site found at 5744
    Illegal NgoMIV site found at 6051
    Illegal NgoMIV site found at 7491
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3298
    Illegal SapI.rc site found at 823
    Illegal SapI.rc site found at 1033
    Illegal SapI.rc site found at 5251
    Illegal SapI.rc site found at 6963


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Categories
Parameters
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