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Coding
Part:BBa_K4740001
Designed by: Andreea Panti Group: iGEM23_CNAS-Romania (2023-10-06)
alkS
This part codes for the alkane-sensing transcription factor alkS. In the presence of alkanes, alkS binds palkB promoter, acting as transcriptional activator of downstream genes. In Pseudomonas putida and Alcanivorax borkumensis, alkS senses the presence of alkanes in the environment and activates transcription of monooxygenase enzymes to allow assimilation of alkanes. Therefore, playing a role, in conjunction with other genes, to degrade oil.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1973
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 726
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 888
Illegal BsaI.rc site found at 359
Illegal BsaI.rc site found at 661
[edit]
Categories
Parameters
None |