Coding

Part:BBa_K4740001

Designed by: Andreea Panti   Group: iGEM23_CNAS-Romania   (2023-10-06)


alkS

This part codes for the alkane-sensing transcription factor alkS. In the presence of alkanes, alkS binds palkB promoter, acting as transcriptional activator of downstream genes. In Pseudomonas putida and Alcanivorax borkumensis, alkS senses the presence of alkanes in the environment and activates transcription of monooxygenase enzymes to allow assimilation of alkanes. Therefore, playing a role, in conjunction with other genes, to degrade oil.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 726
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 888
    Illegal BsaI.rc site found at 359
    Illegal BsaI.rc site found at 661


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Parameters
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