Coding

Part:BBa_K4740000

Designed by: Andreea Panti   Group: iGEM23_CNAS-Romania   (2023-10-06)


alkB1

alkB1 catalyzes the hydroxylation of n-alkanes(C5-C12), allowing oil degradation. This gene has prospective applications in bioremediation of petroleum contaminated waters. This sequence was obtained through codon optimization of the sequence of the alkB1 gene of Alcanivorax borkumensis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1973
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 726
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 888
    Illegal BsaI.rc site found at 359
    Illegal BsaI.rc site found at 661


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Categories
Parameters
None