Plasmid
Part:BBa_K4739014
Designed by: Huaiyuan Ma Group: iGEM23_SUSTech-Shenzhen (2023-10-11)
Knockout plasmid for exoT
ExoT is a gene encoding t3ss exoenzyme, which induces host cell apoptosis. This plasmid is built based on pEXG2.0, a suicide plasmid backbone with sacB, inducing cell lysis when faced with sucrose. It is composed of the homologous arm of exoT. This plasmid is meant for homologous recombination deletion of exoT.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
Illegal EcoRI site found at 1060
Illegal PstI site found at 1646
Illegal PstI site found at 1771 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
Illegal EcoRI site found at 1060
Illegal NheI site found at 3719
Illegal PstI site found at 1646
Illegal PstI site found at 1771 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal EcoRI site found at 1060
Illegal BglII site found at 1755
Illegal BglII site found at 4276
Illegal XhoI site found at 16 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
Illegal EcoRI site found at 1060
Illegal PstI site found at 1646
Illegal PstI site found at 1771 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
Illegal EcoRI site found at 1060
Illegal PstI site found at 1646
Illegal PstI site found at 1771
Illegal NgoMIV site found at 184
Illegal NgoMIV site found at 304
Illegal NgoMIV site found at 400
Illegal NgoMIV site found at 1197
Illegal NgoMIV site found at 1445
Illegal NgoMIV site found at 4612
Illegal NgoMIV site found at 4622
Illegal AgeI site found at 894
Illegal AgeI site found at 996 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1469
Illegal SapI.rc site found at 5890
[edit]
Categories
Parameters
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