Plasmid
Part:BBa_K4739013
Designed by: Huaiyuan Ma Group: iGEM23_SUSTech-Shenzhen (2023-10-11)
Knockout plasmid for exoS
ExoS is a gene encoding t3ss exoenzyme, which induces host cell apoptosis. This plasmid is built based on pEXG2.0, a suicide plasmid backbone with sacB, inducing cell lysis when faced with sucrose. It is composed of the homologous arm of exoS. This plasmid is meant for homologous recombination deletion of exoS.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 7
Illegal PstI site found at 1195
Illegal PstI site found at 1795 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 7
Illegal NheI site found at 3743
Illegal PstI site found at 1195
Illegal PstI site found at 1795 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 7
Illegal BglII site found at 1779
Illegal BglII site found at 4300
Illegal BamHI site found at 821
Illegal XhoI site found at 16
Illegal XhoI site found at 1560 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 7
Illegal PstI site found at 1195
Illegal PstI site found at 1795 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 7
Illegal PstI site found at 1195
Illegal PstI site found at 1795
Illegal NgoMIV site found at 311
Illegal NgoMIV site found at 428
Illegal NgoMIV site found at 544
Illegal NgoMIV site found at 1569
Illegal NgoMIV site found at 4636
Illegal NgoMIV site found at 4646
Illegal AgeI site found at 886 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1444
Illegal SapI.rc site found at 5914
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Categories
Parameters
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