Coding

Part:BBa_K4739007

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-10)


VgrG3-Parkin fusion protein

This part is a fusion protein of VgrG3 from V. cholerae, Parkin, and 3 v5 tag for western blot. VgrG3 proteins puncture the target cell membrane to deliver the toxic effector molecules. Various VgrG proteins might be associated with different effectors and might target different cells, helping the bacteria to deal with various competitive environments and hosts. In T6SS formation of V. cholerae, VgrG3 will form a trimer, assemble to the tip of T6SS and puncture target cell. Testing results can be viewed in BBa_K4739003.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3328
    Illegal PstI site found at 2546
    Illegal PstI site found at 2905
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2546
    Illegal PstI site found at 2905
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3328
    Illegal PstI site found at 2546
    Illegal PstI site found at 2905
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3328
    Illegal PstI site found at 2546
    Illegal PstI site found at 2905
    Illegal NgoMIV site found at 2328
    Illegal NgoMIV site found at 3385
    Illegal AgeI site found at 2179
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None