Coding

Part:BBa_K4739005

Designed by: Huaiyuan Ma   Group: iGEM23_SUSTech-Shenzhen   (2023-10-09)


VgrG3-Dulaglutide fusion protein

This part is a fusion protein of VgrG3 from V. cholerae, Dulaglutide, and 3 v5 tag for western blot. VgrG3 proteins puncture the target cell membrane to deliver the toxic effector molecules. Various VgrG proteins might be associated with different effectors and might target different cells, helping the bacteria to deal with various competitive environments and hosts. In T6SS formation of V. cholerae, VgrG3 will form a trimer, assemble to the tip of T6SS and puncture target cell. Testing results can be viewed in BBa_K4739003.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 2761
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 2761
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 2761
    Illegal NgoMIV site found at 2818
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2299
    Illegal SapI.rc site found at 2736


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Categories
Parameters
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