Composite

Part:BBa_K4733012

Designed by: Diego Perez Hidalgo   Group: iGEM23_UBC-Okanagan   (2023-10-05)


MWPp-RBS-VP3-HisTag-Term

Transcriptional unit for the expression of VP3. This part comes with a His Tag, which allows VP3 to be extracted using His Tag purification. This unit was assembled as a level 1 MoClo part with restriction sites BsaI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 286
    Illegal SpeI site found at 307
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 286
    Illegal SpeI site found at 307
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 286
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 286
    Illegal SpeI site found at 307
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 286
    Illegal SpeI site found at 307
    Illegal NgoMIV site found at 1150
    Illegal AgeI site found at 1063
    Illegal AgeI site found at 1156
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None