![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K4733012
Designed by: Diego Perez Hidalgo Group: iGEM23_UBC-Okanagan (2023-10-05)
MWPp-RBS-VP3-HisTag-Term
Transcriptional unit for the expression of VP3. This part comes with a His Tag, which allows VP3 to be extracted using His Tag purification. This unit was assembled as a level 1 MoClo part with restriction sites BsaI.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 286
Illegal SpeI site found at 307 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 286
Illegal SpeI site found at 307 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 286
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 286
Illegal SpeI site found at 307 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 286
Illegal SpeI site found at 307
Illegal NgoMIV site found at 1150
Illegal AgeI site found at 1063
Illegal AgeI site found at 1156 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |