Composite
Part:BBa_K4733011
Designed by: Diego Perez Hidalgo Group: iGEM23_UBC-Okanagan (2023-10-05)
MWPp-RBS-VP3-HisTag-ThrClLinker-CotB-Term
Transcriptional unit for the expression of VP3 in the outer membrane of vegetative B.Subtilis Cell. The recombinant protein constitutes a His tag for extraction and a Thrombin Cleavable Linker that is able to release VP3 + His Tag into the supernatant. Moreover, the linker connects VP3 and CotB: avoiding the structure disruption of either protein. This unit was assembled as a level 1 MoClo part with restriction sites BsaI.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 286
Illegal EcoRI site found at 1259
Illegal SpeI site found at 307 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 286
Illegal EcoRI site found at 1259
Illegal SpeI site found at 307
Illegal NotI site found at 2239 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 286
Illegal EcoRI site found at 1259 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 286
Illegal EcoRI site found at 1259
Illegal SpeI site found at 307 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 286
Illegal EcoRI site found at 1259
Illegal SpeI site found at 307
Illegal NgoMIV site found at 1150
Illegal AgeI site found at 1063
Illegal AgeI site found at 1156 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1526
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Categories
Parameters
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