Composite

Part:BBa_K4733011

Designed by: Diego Perez Hidalgo   Group: iGEM23_UBC-Okanagan   (2023-10-05)


MWPp-RBS-VP3-HisTag-ThrClLinker-CotB-Term

Transcriptional unit for the expression of VP3 in the outer membrane of vegetative B.Subtilis Cell. The recombinant protein constitutes a His tag for extraction and a Thrombin Cleavable Linker that is able to release VP3 + His Tag into the supernatant. Moreover, the linker connects VP3 and CotB: avoiding the structure disruption of either protein. This unit was assembled as a level 1 MoClo part with restriction sites BsaI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 286
    Illegal EcoRI site found at 1259
    Illegal SpeI site found at 307
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 286
    Illegal EcoRI site found at 1259
    Illegal SpeI site found at 307
    Illegal NotI site found at 2239
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 286
    Illegal EcoRI site found at 1259
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 286
    Illegal EcoRI site found at 1259
    Illegal SpeI site found at 307
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 286
    Illegal EcoRI site found at 1259
    Illegal SpeI site found at 307
    Illegal NgoMIV site found at 1150
    Illegal AgeI site found at 1063
    Illegal AgeI site found at 1156
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1526


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