Part:BBa_K4733007
Photo-cleavable Linker
This coding sequence encodes a 20-long amino acid linker consisting of a photoscission site and a rigid alpha helix-forming linker sequence. The linker is photocleavable when exposed to light with a wavelength of 400 nm.
The sequence has been codon optimized by the 2023 UBC-Okanagan team for expression in the gram-positive model organism, Bacillus Subtilis.
Usage and Biology
The photocleavable incision was designed by Shadish et al. (2019) to have gel-bound fusion protein undergo irreversible photoscission upon exposure to light (wavelength of 400 nm) in a dose-dependent manner and release the bioactive protein.
The UBC-Okanagan 2023 team used the photoscission sequence and fused it on the N-terminus of a rigid linker since we wanted to cut a recombinant protein on that end without having an amino acid residue overhang. A rigid linker was chosen since rigid linkers can separate the functional domains or different proteins within a fusion protein (Chen et al. 2013).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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