Part:BBa_K4728022

TPA detection device (with the transporter TpaK)

This part is a terephthalic acid (TPA) detection device. The TPA-transporter TpaK and regulator (TphR) for the TPA-inducible promoter from Rhodococcus jostii and Comamonas testosteroni, respectively, are constitutively expressed. The reporter, eiraCFP, is under regulation by P_tph, the TPA-inducible promoter that is activated by TPA-bound TphR.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 2829
  Illegal PstI site found at 80
  Illegal PstI site found at 2935
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 2829
  Illegal NheI site found at 958
  Illegal NheI site found at 981
  Illegal PstI site found at 80
  Illegal PstI site found at 2935
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 2829
  Illegal BglII site found at 1591
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 2829
  Illegal PstI site found at 80
  Illegal PstI site found at 2935
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 2829
  Illegal PstI site found at 80
  Illegal PstI site found at 2935
  Illegal AgeI site found at 1363
  
• 1000
  COMPATIBLE WITH RFC[1000]